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Strain Certification
A service of the UNC Mutant Mouse Regional Resource Center
Strain Name: B6;129S-Myh9tm5Rsad
MMRRC ID #: 32096
Date : January 24, 2011
Reference 1 (blue) : C57BL/6J
Reference 2 (red) : TC-1 ES
Gene location: Chr15 77,591,017 bp - 77,672,605 bp
Size of interval containing gene (includes ambiguous regions): 65.95 Mb ( 31.08 Mb - 97.03 Mb)

Notes for B6;129S-Myh9tm5Rsad:

 

Genotyping for MMRRC#32096 was performed on ONE homozygous male mouse that was one generation intrarcrossed within the strain relative to the original mice submitted to the MMRRC-UNC archive by the donating investigator.  When compared to C57BL/6J (Reference 1) and 129S6 (TC-1) (Reference 2) genotypes, MUGA genotyping indicated that the strain background was 74.24% C57BL/6J.   A 65.95Mb region homozygous for 129S6 markers spans the Myh9 locus on chromosome 15.  Based on the single sample that was genotyped, the genetic background of this line is not fixed and contains multiple segregating 129S6 intervals.  The strain detail sheet  did not provide specific information on the degree of heterogeneity in the background.



  Ref 1 (blue) Ref 2 (red) Het Unknown (gray)
Genomic Size  1916.55 Mb  438.78 Mb  226.37 Mb   0.00 Mb
Genomic Fraction  74.24%   17.00%   8.77%   0.00% 
# markers 5861 1309 676 0
informative markers  2248   534   315   
concordant calls  97.55%   94.19%   92.06%   
discordant calls  2.45%   5.81%   7.94%   



Strain Detail Sheet

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