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Strain Certification
A service of the UNC Mutant Mouse Regional Resource Center
Strain Name: B6.129X1-Chrm1tm1Nmn/Mmnc
MMRRC ID #: 11172
Date : January 24, 2011
Reference 1 (blue) : C57BL/6J
Reference 2 (red) : 129X1
Gene location: Chr19 8,738,279 bp - 8,756,057 bp
Size of interval containing gene (includes ambiguous regions): 18.38 Mb ( 0.00 Mb - 18.38 Mb)

Notes for B6.129X1-Chrm1tm1Nmn/Mmnc:

Genotyping for MMRRC#11172 was performed on ONE homozygous male mouse that was obtained by four generations of intrastrain mating relative to the original mice submitted to the MMRRC-UNC archive by the donating investigator.  When compared to C57BL/6J (Reference 1) and 129X1(Reference 2) genotypes, MUGA genotyping indicated that the strain background was 99.25% C57BL/6J.  An interval homozygous for 129X1 markers was identified at the distal end of chromosome 19, adjacent to the Chrm1 locus.  This region should extend through the Chrm1 locus based on PCR genotyping for a homozygous mutation at the locus.  Since the region on Chromosome 19 between 7.6-17.8Mb is IDB between C57BL/6J and 129X1, the recombination location cannot be precisely identified.  Genotyping for this sample is consistent with the information provided by the donating investigator on the strain detail sheet.



  Ref 1 (blue) Ref 2 (red) Het Unknown (gray)
Genomic Size  2562.45 Mb   4.32 Mb   14.93 Mb   0.00 Mb
Genomic Fraction  99.25%   0.17%   0.58%   0.00% 
# markers 7782 13 51 0
informative markers  2953   6   2   
concordant calls  98.44%   83.33%   50.00%   
discordant calls  1.56%   16.67%   50.00%   



Strain Detail Sheet

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