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Strain Certification
A service of the UNC Mutant Mouse Regional Resource Center
Strain Name: B6;129S7-Twist1tm2Bhr/Mmnc
MMRRC ID #: 16842
Date : September 16, 2010
Reference 1 (blue) : C57BL/6J
Reference 2 (red) : 129S7/AB2.2
Gene location: Chr12 34,642,536 bp - 34,644,682 bp
Size of interval containing gene (includes ambiguous regions): 53.00 Mb ( 28.76 Mb - 81.76 Mb)

Notes for B6;129S7-Twist1tm2Bhr/Mmnc:

Genotyping for MMRRC#16842 was performed on ONE homozygous male mouse that was six generations intracrossed within the strain relative to the original mice submitted to the MMRRC-UNC archive by the donating investigator.  When compared to C57BL/6J (Reference 1) and 129S7(Reference 2) genotypes, MUGA genotyping indicated that the strain background was 92.44% C57BL/6J and 2.44% 129S7, including a 53Mb interval homozygous for 129S7 markers on Chromosome 12 spanning the Twist1 locus.  Based on the single sample that was genotyped, the genetic background of this line is not fixed which is consistent with the information provided by the donating investigator on the strain detail sheet. 



  Ref 1 (blue) Ref 2 (red) Het Unknown (gray)
Genomic Size  2386.63 Mb   62.94 Mb  132.13 Mb   0.00 Mb
Genomic Fraction  92.44%   2.44%   5.12%   0.00% 
# markers 7251 194 401 0
informative markers  2765   82   167   
concordant calls  98.59%   98.78%   95.81%   
discordant calls  1.41%   1.22%   4.19%   



Strain Detail Sheet

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© 2010 Leonard McMillan, Alex Jackson and UNC Computational Genetics