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Strain Certification
A service of the UNC Mutant Mouse Regional Resource Center
Strain Name: B6;129S4-F2rl2tm1Cgh
MMRRC ID #: 15230
Date : August 6, 2010
Reference 1 (blue) : C57BL/6J
Reference 2 (red) : 129S4/AK7
Gene location: Chr13 96,466,808 bp - 96,472,694 bp
Size of interval containing gene (includes ambiguous regions): 10.41 Mb ( 90.46 Mb - 100.87 Mb)

Notes for B6;129S4-F2rl2tm1Cgh:

Genotyping for MMRRC#15230 was performed on ONE heterozygous male mouse that was five generations backcrossed onto C57BL/6J relative to the original mice submitted to the MMRRC-UNC archive by the donating investigator.  When compared to C57BL/6J (Reference 1) and 129S4(Reference 2) genotypes, MUGA genotyping indicated that the strain background was 85.82% C57BL/6J.  Based on the single sample that was genotyped, the genetic background of this line is not fixed and contains multiple segregating regions, including a 10.41Mb 129S4 heterozygous interval spanning the F2r2l locus on Chromosome 13.  The mixed strain background for this sample is consistent with the information provided by the donating investigator on the strain detail sheet. 



  Ref 1 (blue) Ref 2 (red) Het Unknown (gray)
Genomic Size  2215.73 Mb   0.00 Mb  365.97 Mb   0.00 Mb
Genomic Fraction  85.82%   0.00%   14.18%   0.00% 
# markers 6740 0 1106 0
informative markers  2588   0   467   
concordant calls  97.18%   0.00%   95.07%   
discordant calls  2.82%   0.00%   4.93%   



Strain Detail Sheet

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